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Oestradiol
Oestradiol is the dominant form of oestrogen in the body and plays a key role in regulating fat distribution and metabolism. In both women and men, abnormal levels of oestradiol can affect body weight by influencing hunger signals, insulin sensitivity, and fat storage—particularly in the hips and abdomen. Fluctuations in oestradiol during menopause or hormonal disorders may lead to weight gain or difficulty losing fat.

Follicle Stimulating Hormone (FSH)
FSH is a hormone that regulates reproductive function but also reflects hormonal ageing. In women, elevated levels are typically seen in perimenopause and menopause, which are associated with shifts in metabolism and an increased tendency to gain weight. In men, low FSH can be linked with hypogonadism, which may impair muscle mass and increase fat accumulation.

HDL % of Total Cholesterol
This percentage shows how much of your total cholesterol is made up of protective HDL cholesterol. A low HDL % is often associated with metabolic syndrome, a cluster of conditions—including abdominal obesity, high blood pressure, and insulin resistance—that increase cardiovascular risk and make weight loss more difficult.

Total Cholesterol, LDL Cholesterol, Non-HDL Cholesterol, Triglycerides
These lipid markers provide a picture of how your body processes fats. Raised LDL and non-HDL cholesterol, along with high triglycerides, are commonly associated with obesity and insulin resistance. Managing these levels is key for both heart health and metabolic function. Elevated levels may indicate that weight gain is impacting cardiovascular health.

HbA1c (Glycated Haemoglobin)
HbA1c measures your average blood sugar control over the past 2–3 months. Elevated HbA1c levels indicate prediabetes or diabetes, both of which can hinder weight loss by disrupting insulin function and promoting fat storage. Research from the NHS Diabetes Prevention Programme confirms the link between glycaemic control and weight management outcomes.

Free Triiodothyronine (FT3), Free Thyroxine (FT4), Thyroid Stimulating Hormone (TSH)
These three markers assess thyroid function, which is central to metabolic rate. An underactive thyroid (hypothyroidism) can cause fatigue, cold intolerance, and weight gain due to reduced basal metabolic activity. Even subclinical thyroid dysfunction can stall weight loss, which is why this full panel is essential for metabolic screening.

Cortisol
Cortisol, the primary stress hormone, promotes fat storage—especially visceral (belly) fat—when chronically elevated. Persistently high levels are often a result of psychological stress, overtraining, or sleep deprivation. Cortisol also contributes to insulin resistance and cravings for high-calorie foods, making it a key focus in stubborn weight cases.

Alkaline Phosphatase (ALP), Gamma-glutamyltransferase (GGT), Alanine Transferase (ALT), Aspartate Aminotransferase (AST)
These liver enzymes help evaluate liver health and screen for non-alcoholic fatty liver disease (NAFLD), which is common in individuals with obesity. Poor liver function can impair fat metabolism and toxin clearance, reducing the body’s ability to lose weight efficiently.

Albumin, Total Protein
These proteins reflect your nutritional status and overall health. Albumin in particular is a marker of protein intake and liver synthesis capacity. Low levels may indicate malnutrition, chronic inflammation, or liver dysfunction, all of which can influence weight and energy levels.

hs-CRP (high-sensitivity C-Reactive Protein)
This is a highly sensitive measure of systemic inflammation. Raised hs-CRP levels are associated with insulin resistance, cardiovascular risk, and obesity. Inflammation can also impair muscle recovery and slow metabolism, contributing to weight retention despite diet and exercise.

Testosterone
In both sexes, testosterone affects muscle mass, fat distribution, and energy levels. Low testosterone in men, or imbalances in women, can lead to increased body fat—particularly in the abdomen—and reduced muscle tone. It also plays a role in motivation and stamina, indirectly influencing physical activity levels.

Iron
Iron supports red blood cell production and oxygen transport. Deficiency can lead to fatigue and reduced physical performance, which can undermine your ability to exercise consistently and lose weight. Low iron is common, especially in menstruating women or those on restrictive diets.

Vitamin D
Vitamin D plays a role in fat metabolism, immune regulation, and insulin sensitivity. Numerous studies, including those published in the British Journal of Nutrition, have linked low vitamin D levels to obesity and poor metabolic health. Supplementation may improve weight loss outcomes when deficiency is identified.

Bilirubin
Bilirubin reflects the breakdown of red blood cells and is processed by the liver. Abnormal levels can signal liver dysfunction or haemolysis, both of which can impact metabolic processes relevant to weight regulation.

Creatine Kinase
Creatine kinase is an enzyme that reflects muscle activity and damage. Elevated levels may result from intense exercise or muscle injury. Monitoring CK can help ensure your exercise programme is optimally tailored—maximising fat burn while avoiding overtraining and muscle breakdown.