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Testosterone
Testosterone is an essential androgen hormone predominantly produced in the testes in men and, to a lesser extent, in the ovaries and adrenal glands in women. It regulates libido, muscle mass, bone density, mood, and red blood cell production. In clinical contexts, testosterone is often measured to diagnose androgen deficiency, particularly in men presenting with low energy, reduced sexual desire, or loss of lean muscle. Research from institutions such as King’s College London has linked declining testosterone levels with increased risk of cardiovascular disease, type 2 diabetes, and cognitive decline in ageing males. Conversely, abnormally high levels may point to the use of anabolic steroids or the presence of hormone-secreting tumours. Its inclusion in this panel helps assess whether the body is in an anabolic state, guiding treatment options such as hormone replacement therapy or lifestyle adjustments.

Cortisol
Cortisol is a glucocorticoid hormone produced by the adrenal cortex, essential for regulating metabolism, immune response, and the body’s ability to manage stress. It follows a daily rhythm, peaking in the morning and declining throughout the day. Persistently elevated cortisol levels can result from chronic stress or conditions like Cushing’s syndrome, and may lead to issues such as abdominal weight gain, high blood pressure, insomnia, and suppressed immunity. On the other hand, low cortisol levels may indicate adrenal insufficiency or Addison’s disease, which can manifest with symptoms such as low energy, dizziness, and electrolyte imbalances. Measuring cortisol alongside testosterone allows for a more complete picture of endocrine health, especially in assessing the catabolic–anabolic balance that is critical for recovery, mood regulation, and metabolic function.